Fully Codon-Optimized luciferase Uncovers Novel Temperature Characteristics of the Neurospora Clock
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چکیده
منابع مشابه
Fully codon-optimized luciferase uncovers novel temperature characteristics of the Neurospora clock.
We report the complete reconstruction of the firefly luciferase gene, fully codon optimized for expression in Neurospora crassa. This reporter enhances light output by approximately 4 log orders over that with previously available versions, now producing light that is visible to the naked eye and sufficient for monitoring the activities of many poorly expressed genes. Time lapse photography of ...
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15 صفحه اولThe Molecular Clock of Neurospora crassa
1988 Ph.D. University of Heidelberg, Germany 1989 1991 PostDoc Princeton University and Rockefeller Research Laboratory, New York, USA (Prof. James E. Rothman) 1992 1998 Group Leader and habilitation Ludwig-MaximiliansUniversität München, Germany (Prof. Walter Neupert) 1998 2000 Professor Ludwig-Maximilians-Universität München, Germany since 2000 Full Professor BZH 2010 2013 Director BZH
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Neurospora crassa has been systematically investigated for circadian entrainment behavior. Many aspects of synchronization can be investigated in this simple, cellular system, ranging from systematic entrainment and drivenness to masking. Clock gene expression during entrainment and entrainment without clock genes suggest that the known transcription/translation feedback loop is not alone respo...
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The eukaryotic circadian clocks are composed of autoregulatory circadian negative feedback loops that include both positive and negative elements. Investigations of the Neurospora circadian clock system have elucidated many of the basic mechanisms that underlie circadian rhythms, including negative feedback and light and temperature entrainment common to all eukaryotic clocks. The conservation ...
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ژورنال
عنوان ژورنال: Eukaryotic Cell
سال: 2008
ISSN: 1535-9778,1535-9786
DOI: 10.1128/ec.00257-07